Fabrication of a monolithic polymer layer, as the stationary phase, equipped with two electrodes was studied for simultaneous two-dimensional separation, combining high performance liquid chromatography and orthogonal electrophoresis. The monolithic polymer layer was prepared between a pair of glass plates by a two-step UV-initiated photo-polymerization. Prior to preparation of the monolithic polymer layer for separation, high-density monolith bars were prepared along the electrodes to suppress the penetration of bubbles formed at the electrodes into the separation monolith. Using the monolithic polymer layer, 2D separation of colored-dyes was successfully achieved by combining gradient HPLC and orthogonal electrophoresis. The 2D separation of proteins was also demonstrated.
2016 The Society for Chromatographic Sciences