CHROMATOGRAPHY
Online ISSN : 1348-3315
Print ISSN : 1342-8284
Original papers
Sleep-Awake Profile Related Circadian D-Alanine Rhythm in Human Serum and Urine
Akiko MORIKAWAHideoki FUKUOKAKeiko UEZONOMasashi MITASatoru KOYANAGIShigehiro OHDOKiyoshi ZAITSUKenji HAMASE
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JOURNALS FREE ACCESS

2017 Volume 38 Issue 2 Pages 53-58

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Abstract

The circadian profiles of D-alanine (Ala) amounts in human serum and urine have been clarified using a two-dimensional HPLC system combining a microbore reversed-phase C18 column and a Pirkle-type enantioselective column. The two-dimensional HPLC system enables the determination of trace amounts of D-Ala in the clinical samples without severe interference from intrinsic substances in the human physiological fluids. In the volunteers having normal sleep-awake patterns, the amounts of D-Ala in the serum were high in the late evening and low in the morning. On the other hand, in the volunteers having the reversed sleep-awake patterns, the amounts of D-Ala in the serum were high in the morning and low in the nighttime. The similar circadian changes of D-Ala amounts were observed in the urine. These 24-h profiles of D-Ala are almost the same as those observed in rats and mice, suggesting that D-Ala has fundamental physiological functions related to rest-active conditions in mammals, and could also be useful as the biomarker for sleep-awake profiles.

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© 2017 The Society for Chromatographic Sciences
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