CHROMATOGRAPHY
Online ISSN : 1348-3315
Print ISSN : 1342-8284
Original Papers
Quantitative Analysis of Clenbuterol in Pig Liver by LC-MS/MS/MS
Takako HAYASHITeruhisa FUJIMAKIMaki MIYAZAWA
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2018 Volume 39 Issue 3 Pages 131-137

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Abstract

We examined the utility of LC-MS/MS/MS in the rapid quantitative analysis of clenbuterol in pig liver. Compared with LC-MS/MS in the SRM mode, which gave a chromatogram of pig liver containing interference peaks near the target component peak, LC-MS/MS/MS generated a clear chromatogram with no interference peaks. Validation of the method yielded favorable results for both LC-MS/MS in the SRM mode and LC-MS/MS/MS, with trueness values of 95.5% and 102%, repeatability of 1.9% and 5.5%, and within-laboratory reproducibility of 2.0% and 5.1%, respectively. Moreover, the recovery of clenbuterol-d9 used as surrogate was ≥70% in both measurement methods and thus, validation was achieved in terms of both selectivity and limit of quantification. The results suggest that LC-MS/MS/MS has highly selectivity for quantitative analysis as the influence of interference peaks is suppressed, and can be used when quantification is difficult with LC-MS/MS in the SRM mode due to matrix effects.

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© 2018 The Society for Chromatographic Sciences
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