CHROMATOGRAPHY
Online ISSN : 1348-3315
Print ISSN : 1342-8284
Original Papers
On-Line Derivatization and Concentration of Aspartic Acid Using In Situ Photopolymerized Carboxylic Acid Type Polyacrylamide Gels as a Permselective Preconcentrator
Sachio YAMAMOTONoriaki NISHIDAMitsuhiro KINOSHITAShigeo SUZUKI
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2018 Volume 39 Issue 3 Pages 125-130

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Abstract

Herein, we report a method for the simultaneous derivatization and concentration of aspartic acid using an acidic polyacrylamide gel, fabricated in the microfluidic channel of a commercial poly(methyl methacrylate) (PMMA) microchip. This approach is based on a simple photochemical copolymerization for the fabrication of the anionic polyacrylamide gel. The intersection of the PMMA microchip was filled with a solution containing acrylamide, N,N'-methylene-bis-acrylamide, 2-acrylamidoglycolic acid, and riboflavin as photoinitiator, which functioned as a photocatalytic initiator. In situ polymerization near the cross of the sample outlet channel was performed by pinpoint irradiation with an argon ion laser beam, which is also used as the light source for fluorimetric detection. The electrokinetic properties, combined with the electrostatic repulsion between both sample and reagent components with the anionic groups in the polyacrylamide gel, enable simultaneous derivatization and entrapment at the cathodic side of the gel plug.

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© 2018 The Society for Chromatographic Sciences
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