2018 Volume 39 Issue 3 Pages 153-160
This paper describes simultaneous separation of six active ingredients, 6-ginger, 8-ginger, 10-ginger, 6-shogaol, honokiol, and magnolol, contained in ginger, processed ginger and magnolia bark by reversed-phase HPLC with core-shell (CS) type columns. Four different CS columns, Bipheny, Phenylhexyl, Cholester and typical C18 columns were employed. Among four CS columns, successful separation of these six components was achieved by a Biphenyl column within 5 min, using acetonitrile 50% mobile phase. The method was then applied for the assay of these active ingredients in Kampo products (extract granules), which are prepared according to the prescription using more than five crude drugs. 6-Gingerol and 6-shogaol were successfully separated from the other components in Kampo products, and a fast assay within 10 min was achieved in all four Kampo products tested. Two active ingredients contained in magnolia bark were also successfully separated and determined by the method in all four Kampo products tested within 10 min.