CHROMATOGRAPHY
Online ISSN : 1348-3315
Print ISSN : 1342-8284
ISSN-L : 1342-8284
Original Papers
LVSEP Analysis of Cationic Analytes in Cationic Polymer-Coating Microchannel Prepared by Vacuum-Drying Method
Fumihiko KITAGAWA Yuka NIIMIYAIsoshi NUKATSUKA
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2021 Volume 42 Issue 2 Pages 109-114

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Abstract

To improve the detection sensitivity of cationic analytes with simple experimental procedures in microchip electrophoresis (MCE), large-volume sample stacking with an electroosmotic flow (EOF) pump (LVSEP) was performed in polymer-coated microchannels. At first, cationic polybrene (PB) was employed as the coating polymer to reverse the EOF. In the LVSEP analyses, however, the PB-coated microchannel gave insufficient enrichments and broader peaks for cationic standard dyes. By the application of neutral poly(vinyl alcohol) and cationic poly(allylamine) mixture-coating, on the other hand, good enrichments of histamine were achieved by LVSEP with a sensitive enhancement factor of 120.

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