2024 Volume 45 Issue 3 Pages 139-144
Monoacylglycerols (MAGs) are one of the class of glycerides which are composed of a molecule of glycerol linked to a fatty acid via an ester bond. In addition to roles of MAGs as membrane components and energy storages, several MAGs are well-known as a signal mediator. In the present study, authors investigate the mass spectrometry (MS) conditions in the positive ion mode for 10 MAG species to obtain information on sensitivity derived from the combination of the precursor ion and the product ion, and differences in the ionization efficiency of MAG species in liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. Based on studies utilizing a 17:0-MAG standard substance, the combination of an ammonium adduct ion and a dehydroxylated ion showed higher sensitivity compared with other combinations. However, examinations with other MAGs revealed that the slope of the calibration curve varied depending on both the carbon chain length and the desaturation index of acyl groups. Finally, the difference in the calibration slope values was utilized as a correction parameter for the quantification of MAG contents in human lung carcinoma H292 cells to clarify its sensitivity for influenza A virus (IAV) infection. Obtained results suggest that the progression of IAV-infection might disturb intracellular lipid homeostasis, leading to an increase of MAG levels.
