LVSEP Analysis of Phosphopeptides in Dynamically PVP-Coated Capillaries and Microchannels

Fumihiko KITAGAWA; Ayaka HAYASHI; Isoshi NUKATSUKA

doi:10.15583/jpchrom.2021.018

This article has now been updated. Please use the final version.

LVSEP Analysis of Phosphopeptides in Dynamically PVP-Coated Capillaries and Microchannels

Fumihiko KITAGAWA , Ayaka HAYASHI, Isoshi NUKATSUKA

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Keywords: Capillary electrophoresis, Microchip electrophoresis, On-line sample preconcentration, LVSEP, Dynamic coating, Phosphopeptides

JOURNAL FREE ACCESS Advance online publication

Article ID: 2021.018

DOI https://doi.org/10.15583/jpchrom.2021.018

The final version of this article is now available: Vol. 43 (2022), No. 1 pp. 37-41

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Abstract

To achieve highly sensitive analyses of phosphopeptides with simple experimental procedures in capillary electrophoresis (CE) and microchip electrophoresis (MCE), large-volume sample stacking with an electroosmotic flow pump (LVSEP) was performed in dynamically poly(vinyl pyrrolidone) (PVP)-coated capillaries to suppress the electroosmotic flow. In the analysis of monophosphopeptide (MPP) from bovine β-casein, good enrichments were attained with the sensitive enhancement factor (SEF) of 260 and 400 in CE and MCE, respectively, by using 0.2% PVP in 20 mM HEPES buffer (pH 9.0) in LVSEP.

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