Data for proteomic analysis of in situ digestion of alcohol-fixed cells for quantitative proteomics

Abstract

We have developed a novel method for the preparation of proteome samples by direct digestion of methanol-fixed cells with trypsin, named in situ digestion of alcohol-fixed cells for quantitative proteomics (iSDAC). In this method, the cells are fixed in methanol and digested directly with trypsin without solubilizing cells with detergents. The elimination of the detergent removal step allows for easy and rapid sample preparation. This method’s digestion efficiency and reproducibility are comparable to those of commonly used proteome sample preparation methods.