Dataset for proteomic analysis of secreted proteins from bovine periosteum and blood vessels with explant culture

Abstract

A combination of mass spectrometry (MS) and immunohistochemistry was used to detect important proteins for bone regeneration. In a previous study, electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) MS discovered candidate proteins from supernatants of bovine periosteum, then immunohistochemistry revealed protein expression in periosteum tissue. Using these methods, F-box and WD-40 domain-containing protein 2 (FBXW2) was found in elastic fibers in the periosteum and blood vessels. Osteocalcin was expressed around FBXW2 in the periosteum. However, the combination of ESI-Q-TOF MS and immunohistochemistry revealed expression of several proteins including FBXW2, uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), exosome complex component RRP45 (EXOSC9), thioredoxin-related transmembrane protein 2 (ΤΜX2), and F-box/leucine-rich repeat protein 14 (FBXL14). In the present study, proteins in supernatants of the periosteum were investigated using ESI-Q-Orbitrap MS and then compared with the results obtained from blood vessels. Tissue specific proteins were compared between the periosteum and blood vessels, especially components of elastic fibers. ESI-Q-Orbitrap MS detected over 890 similar proteins secreted from both the periosteum and blood vessel and other proteins secreted exclusively from only the periosteum or blood vessels. ESI-Q-TOF MS and ESI-Q-Orbitrap MS detected collagen types 1 and 3, but only ESI-Q-Orbitrap MS detected collagen type 4.