Distribution of DJ-1, Parkinson’s Disease-Related Protein PARK7, and Its Alteration in 6-Hydroxydopamine-Treated Hemiparkinsonian Rat Brain

Takashi Yanagida; Kazuyuki Takata; Masatoshi Inden; Yoshihisa Kitamura; Takashi Taniguchi; Kanji Yoshimoto; Takahiro Taira; Hiroyoshi Ariga

doi:10.1254/jphs.SC0060098

Short Communications

Distribution of DJ-1, Parkinson’s Disease-Related Protein PARK7, and Its Alteration in 6-Hydroxydopamine-Treated Hemiparkinsonian Rat Brain

Takashi Yanagida, Kazuyuki Takata, Masatoshi Inden, Yoshihisa Kitamura, Takashi Taniguchi, Kanji Yoshimoto, Takahiro Taira, Hiroyoshi Ariga

Author information

Takashi Yanagida
Department of Neurobiology and 21st Century COE Program, Kyoto Pharmaceutical University, Japan
Kazuyuki Takata
Department of Neurobiology and 21st Century COE Program, Kyoto Pharmaceutical University, Japan
Masatoshi Inden
Department of Neurobiology and 21st Century COE Program, Kyoto Pharmaceutical University, Japan
Yoshihisa Kitamura
Department of Neurobiology and 21st Century COE Program, Kyoto Pharmaceutical University, Japan
Takashi Taniguchi
Department of Neurobiology and 21st Century COE Program, Kyoto Pharmaceutical University, Japan
Kanji Yoshimoto
Department of Legal Medicine, Kyoto Prefectural University of Medicine, Japan
Takahiro Taira
Department of Molecular Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, Yamanashi University, Japan
Hiroyoshi Ariga
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Japan

Keywords: DJ-1, 6-hydroxydopamine, Parkinson’s disease

JOURNAL FREE ACCESS

2006 Volume 102 Issue 2 Pages 243-247

DOI https://doi.org/10.1254/jphs.SC0060098

View "Advance Publication" version (October 12, 2006).

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Abstract

DJ-1 has multiple functions and its dysfunction may be linked to the onset of familial Parkinson’s disease PARK7. However, the function and distribution of DJ-1 is unclear. In this study, we determined DJ-1 distribution and change after intranigral injection of 6-hydroxydopamine (6-OHDA). Although distribution of DJ-1 immunoreactivity was not changed in cerebral cortex and striatum, 6-OHDA caused increase of DJ-1 in the particulate fraction and decrease in the cytosolic fraction in substantia nigra. At that time, DJ-1 shifted to acid forms. These results suggest that distributional changes, translocation, and acidic shift of DJ-1 may be compensatory responses to protect against 6-OHDA-induced oxidative stress.

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