2007 Volume 104 Issue 1 Pages 108
Wrong:Oligonucleotide primer pairs specific for the respective TRP isoforms were as follows: TRPC6F: GGCCTCATGATTATTTCTGCAAGT and TRPC6R: GCCTTCAAATCTGTCAGCTGCA, TRPM4F: GTATGCCAACTGGCTGGTGGT and TRPM4R: GTCCACCTTCTGGGACGTGC, TRPV4F: CCCCCATCCTCAAAGTCTTCAA and TRPV4R: GGGCAGCTCCCCAAAGTAGAA.
Right:Oligonucleotide primer pairs specific for the respective TRP isoforms were as follows: TRPC6F: CATCCCAGTGGTGCGGAAGA and TRPC6R: GCCTTCAAATCTGTCAGCTGCA, TRPM4F: GTCATCGTGAGCAAGATGATGAA and TRPM4R: GTCCACCTTCTGGGACGTGC, TRPV4F: GTGCCTGGGCCCAAGAAA and TRPV4R: GGGCAGCTCCCCAAAGTAGAA. For nested PCR, each of the forward primers was changed from that used in the initial PCR, and the sequences of the nested forward primers were as follows: TRPC6NF: GGCCTCATGATTATTTCTGCAAGT, TRPM4NF: GTATGCCAACTGGCTGGTGGT, and TRPV4NF: CCCCCATCCTCAAAGTCTTCAA.