2007 Volume 104 Issue 1 Pages 46-55
Amyloid β (Aβ) toxicity has been implicated in cell death in the hippocampus, but its specific mechanisms are poorly understood. In this study, Aβ-induced cell death was investigated in organotypic hippocampal slice cultures (OHCs) that were cultured for various periods in vitro. There were no obvious histological differences among slices cultured for 3 to 7 weeks in vitro. Although there was little neurotoxicity after treatment with Aβ25–35 in OHCs cultured for relatively shorter periods (3 – 5 weeks), age-dependent cell death was evident in OHCs cultured for relatively longer periods (6 – 7 weeks) after exposure to Aβ25–35. In OHCs cultured for 7 weeks, S-allyl-