Journal of Pharmacological Sciences
Short Communications
A Low-Cost Method for Brain Slice Cultures
Ryuta KoyamaRieko MuramatsuTakuya SasakiRie KimuraChihiro UeyamaMakoto TamuraNaohiro TamuraJunya IchikawaNaoya TakahashiAtsushi UsamiMaki K. YamadaNorio MatsukiYuji Ikegaya
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Volume 104 (2007) Issue 2 Pages 191-194

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Abstract

Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM® method.

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© The Japanese Pharmacological Society 2007
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