Honeybee Royal Jelly and Nobiletin Stimulate CRE-Mediated Transcription in ERK-Independent and -Dependent Fashions, Respectively, in PC12D Cells

Hironori Fujiwara; Ako Kogure; Masahiro Sakamoto; Tohru Yamakuni; Yoshihiro Mimaki; Kiyoshi Murata; Nobuyuki Hitomi; Kikuji Yamaguchi; Yasushi Ohizumi

doi:10.1254/jphs.11001SC

Short Communications

Honeybee Royal Jelly and Nobiletin Stimulate CRE-Mediated Transcription in ERK-Independent and -Dependent Fashions, Respectively, in PC12D Cells

Hironori Fujiwara, Ako Kogure, Masahiro Sakamoto, Tohru Yamakuni, Yoshihiro Mimaki, Kiyoshi Murata, Nobuyuki Hitomi, Kikuji Yamaguchi, Yasushi Ohizumi

Author information

Hironori Fujiwara
Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Japan
Ako Kogure
Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Japan
Masahiro Sakamoto
Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Japan
Tohru Yamakuni
Laboratory of Pharmacotherapy, Graduate School of Pharmaceutical Sciences, Tohoku University, Japan
Yoshihiro Mimaki
Laboratory of Medicinal Plant Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
Kiyoshi Murata
Japan Royal Jelly (JRJ) Co., Ltd., R&D Center, Japan
Nobuyuki Hitomi
Japan Royal Jelly (JRJ) Co., Ltd., R&D Center, Japan
Kikuji Yamaguchi
Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Japan
Japan Royal Jelly (JRJ) Co., Ltd., Japan
Apis Cerana Research Institute, Yunnan Agriculture University, China
Yasushi Ohizumi
Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Japan
Yokohama College of Pharmacy, Japan
Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, Japan

Keywords: honeybee royal jelly, CRE-mediated transcription, Alzheimer’s disease

JOURNAL FREE ACCESS

2011 Volume 116 Issue 4 Pages 384-387

DOI https://doi.org/10.1254/jphs.11001SC

View "Advance Publication" version (July 9, 2011).

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Abstract

To prove the pharmacological actions of honeybee royal jelly (RJ) on the nervous system, we examined the effects of RJ on CRE-mediated transcription. RJ increased CRE-mediated transcription in PC12D cells. Moreover, CRE-mediated transcriptional activity by RJ was enhanced by nobiletin. U0126, a MEK inhibitor, inhibited CRE-mediated transcription by combining RJ plus nobiletin without affecting transcription by RJ alone. These results suggest that RJ stimulates CRE-mediated transcription via an ERK-independent cascade, whereas the increasing CRE-mediated transcriptional effect by nobiletin is dependent on ERK phosphorylation. Combining RJ plus nobiletin may activate effectively neuronal functions via enhancement of CRE-mediated transcription.

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