Journal of Pharmacological Sciences
Online ISSN : 1347-8648
Print ISSN : 1347-8613
ISSN-L : 1347-8613
Short Communications
Formaldehyde-Induced Shrinkage of Rat Thymocytes
Hiromi NakaoChisato UmebayashiMami NakataYasutaka NishizakiKatuhiko NodaYoshiro OkanoYasuo Oyama
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2003 Volume 91 Issue 1 Pages 83-86


To test the possibility that micromolar formaldehyde, a metabolite of methanol derived from aspartame, exerts cytotoxicity, its effect on rat thymocytes was examined under the in vitro condition using a flow cytometer. Incubation of thymocytes with formaldehyde at 100 μM or more for 24 h significantly increased the populations of shrunken cells and cells with hypodiploid DNA. The peak blood concentration of methanol in human subjects administered abuse doses of aspartame has been reported to exceed 2 mg/dL (625 μM). It would increase the population of thymocytes undergoing apoptosis if formaldehyde at 100 μM or more appears in the blood after administration of aspartame.

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© The Japanese Pharmacological Society 2003
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