α-Phenyl-N-tert-butyl Nitrone Has Scavenging Activity Against Singlet Oxygen (1O2) and Attenuates 1O2-Induced Neuronal Cell Death

Anna Kawai; Yoko Nishinaka; Toshiyuki Arai; Kiichi Hirota; Hiroko Mori; Nobuyuki Endo; Takashi Miyoshi; Kouhei Yamashita; Masataka Sasada

doi:10.1254/jphs.08233SC

This article has now been updated. Please use the final version.

α-Phenyl-N-tert-butyl Nitrone Has Scavenging Activity Against Singlet Oxygen (¹O₂) and Attenuates ¹O₂-Induced Neuronal Cell Death

Anna Kawai, Yoko Nishinaka, Toshiyuki Arai, Kiichi Hirota, Hiroko Mori, Nobuyuki Endo, Takashi Miyoshi, Kouhei Yamashita, Masataka Sasada

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Keywords: α-phenyl-N, butyl nitrone, singlet oxygen, neuronal cell death

JOURNAL FREE ACCESS Advance online publication

Article ID: 08233SC

DOI https://doi.org/10.1254/jphs.08233SC

The final version of this article is now available: Vol. 108 (2008) , No. 4 p.545

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Abstract

Scavenging activity of α-phenyl-N-tert-butyl nitrone (PBN) against singlet oxygen (¹O₂) and its effects on ¹O₂-induced neuronal cell death were examined. PBN at 1 – 4 mM dose-dependently suppressed ¹O₂ released from activated human neutrophils. PBN did not react with hydrogen peroxide or hypochlorite and did not affect myeloperoxidase activity, which are involved in the ¹O₂ formation in neutrophils. PBN also suppressed chemically generated ¹O₂ in a cell-free system. These findings collectively indicated that PBN certainly has scavenging activity against ¹O₂. Furthermore, PBN attenuated ¹O₂-induced neuronal cell death. The well-known neuroprotective effects of PBN might be attributed to its ¹O₂-scavenging activity.

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