Edaravone, a Radical Scavenger, Inhibits Mitochondrial Permeability Transition Pore in Rat Brain

Abstract

Edaravone, a radical scavenger, prevents ischemia/reperfusion injury in the brain, but the detailed mechanism is not known. This study examines the effect of edaravone on mitochondrial permeability transition pore (PTP) in rat brain. Edaravone at 10 – 100 μM inhibited Ca²⁺- and H₂O₂-induced swelling of mitochondria isolated from rat brain. Addition of Ca²⁺ generated reactive oxygen species (ROS) in isolated mitochondria. Edaravone (10 – 100 μM) inhibited Ca²⁺-induced generation of ROS. These results suggest that edaravone inhibits opening of mitochondrial PTP in the brain, and they imply that inhibition of mitochondrial PTP may account for the neuroprotective effect of edaravone.