QUANTITATIVE EXTRACTION OF EVANS BLUE LEAKED FROM THE VESSELS IN THE SKIN

Abstract

Several investigators have used the extravasal leakage of the circulating dye as an indicator of the change in capillary permeability by measuring, for example, the mean diameter of the blueing (1-3), the visual grading of the blueing (4, 5) or size and intensity of the staining (6-12). Studying the effects of organic solvents on the capillary permeability, one of the present authors has indicated the need for the more exact and quantitative evaluation of the leaked dye (7) and attemped further the extraction of the tissue dye by butanol (13). The quantitative extraction of the dye has been developed by Beach and Steinetz (14), Judah and Willoughby (15) and Nitta et al. (16). However, the procedures except that introduced by Beach and Steinetz, were relatively complicated for use as a routine bioassay in the laboratory. The present report is a device to obtain the more simple method available in the laboratory by modifying the previous method.