SEPARATION OF CATECHOLAMINES ON THE PHOSPHO-CELLULOSE COLUMN

Abstract

Metanephrine, adrenaline (together with tyramine and normetanephrine) and noradrenaline (together with dopamine) were separated by a simple chromatographic procedure on a phospho-cellulose column. The elution was carried out with 0.025M phosphate buffer (pH 6.2) containing EDTA. The method was successfully applied to neutralized perchloric acid extract of biological material. Separations of other amines (tryptamine, serotonin, histamine, agmatine, putrescine, cadaverine, spermidine and spermine) and basic amino acids (lysine, histidine and arginine) also were achieved utilizing the same column.