The Japanese Journal of Pharmacology
EFFECT OF PYRIDOXAL PHOSPHATE DEFICIENCY ON AROMATIC L-AMINO ACID DECARBOXYLASE ACTIVITY WITH L-DOPA AND L-5-HYDROXYTRYPTOPHAN AS SUBSTRATES IN RATS
Mohammed Khalilur RAHMANToshiharu NAGATSUTae SAKURAISeiji HORIMiyako ABEMakoto MATSUDA
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Volume 32 (1982) Issue 5 Pages 803-811

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Abstract

This paper describes the distribution of aromatic L-amino acid decarboxylase (AADC) activities in fourteen tissues (eight peripheral tissues and six brain regions) of semicarbazide (SC)-treated rats, using both LDOPA and L-5-hydroxytryptophan (L-5-HTP) as substrates. The distribution of pyridoxal phosphate (PLP) was also measured in control and SC-treated rats. SC-treatment decreased the PLP concentration in all tissues (about 50-60% of control). AADC activities towards L-DOPA and L-5-HTP as substrates were also decreased significantly in almost all tissues of SC-treated rats. After the addition of exogenous PLP in vitro, AADC activities were recovered only partially in most tissues, but the recovery patterns were not parallel between L-DOPA and L-5-HTP as substrates. L-DOPA decarboxylase activity was more sensitive to PLP-deficiency than L-5-HTP decarboxylase activity in the same tissues. Serum AADC activities were decreased drastically using both L-DOPA and L-5-HTP as substrates. No serum AADC activity was detected in SC-treated rats using L-DOPA as substrate, but low activity was detected in the same sample using L-5-HTP as the substrate; both activities recovered completely after in vitro addition of 10μM PLP in the incubation mixtures.

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