Defense Mechanisms against Cadmium Toxicity

I. A Biochemical and Histological Study of the Effects of Pretreatment with Cadmium on the Acute Oral Toxicity of Cadmium in Mice

Abstract

Resistance to the lethal action of Cd²⁺ produced in mice was maintained for 6 to 24 hr after pretreatment with 1/10 of the challenge Cd²⁺ doses as shown by an increased oral LD50. Pretreatment 24 hr prior to the challenge doses was most effective. In addition, 1/20 to 1/7 of the challenge doses was necessary to acquire the tolerance to the acute oral toxicity of Cd²⁺. The largest effect was found for pretreatment with 1/7 of the challenge doses. Acute liver injury at 24 hr after challenge with a large dose of Cd²⁺ (100 mg Cd2+/kg, p.o.) was markedly reduced by pretreatment with a small dose of the cation (15 mg Cd2+/kg, p.o.) 24 or 48 hr prior to the challenge dose as shown by a marked reduction in serum GOT and GPT activities and the reversal of histopathological changes. The elevated serum urea nitrogen at 4 hr after the Cd²⁺ challenge was reduced by pretreatment 6 to 24 hr prior to the challenge dose. In spite of the increased urea nitrogen 4 hr after the Cd²⁺ challenge, no morphological alterations were observed in the kidney at 24 hr. Serum Alp activity was not significantly influenced by the Cd²⁺ challenge. Glucose in serum was increased by the administration of a small dose of Cd²⁺, but was unaffected by a large dose. Decreases in hepatic RNA and DNA concentrations at 24 hr after the Cd²⁺ challenge were prevented by pretreatment 24 or 48 hr prior to the challenge dose. Potentiation of hexobarbital sleep time was observed at 6 or 24 hr after a small dose of Cd²⁺. Nevertheless, further potentiation at 24 hr after the Cd²⁺ challenge (75 mg Cd²⁺/kg, p.o., in this case) was reduced by pretreatment 24 hr prior to the challenge dose. A major target organ for the acute oral toxicity of Cd²⁺ was the liver rather than the kidney.