Effect of 2, 4, 6-Trinitrophenol on Catecholamine Secretion from Perfused Bovine Adrenal Glands

Abstract

2, 4, 6-Trinitrophenol (PA) evoked a prolonged catecholamine (CA) secretion from perfused bovine adrenal glands. The PA-evoked CA secretion was concentration-dependent, required the presence of extracellular calcium and resulted from a direct action of PA on the chromaffin cells. Furthermore, PA reduced Mg²⁺-ATPase activities in the plasma membrane-rich microsome and granule-rich fraction from the adrenal medulla. These results indicate that PA evokes CA secretion through the actions on both the chromaffin cell membranes and Granule membranes.