Abstract
A new simple and economical plastic Boyden chamber for in vitro assay of leukocytes chemotaxis was devised by the use of a commercially available immunoassay plate. Zymosan-activated rat serum, formly-methionyl-leucylphenylalanine and leukotriene B4 caused concentration-dependent migration of the leukocytes into the lower chamber. About 50% of the cells loaded in the upper chamber migrated into the lower chamber for 80 min at the optimal concentrations of the three chemoattractants tested.
