Abstract
Pretreatment of cerebral synaptic membranes with arachidonic acid (10 nM—1 μM) at 37°C for 40 min caused a significant increase in [3H]clonidine binding but pretreatment at 4°C did not. Scatchard analysis showed that 100 nM arachidonic acid pretreatment resulted in a significant elevation of the Bmax value of the low affinity site. PGD2, PGE2 and PGF2α (10-100 μM) pretreatment also enhanced binding. These polyunsaturated fatty acids could be involved in regulation of the α2-adrenoceptor binding in rat cerebral cortical membranes.
