Abstract
Extracellular ATP (adenosine 5'-triphosphate) produces a dose-dependent elevation of DNA synthesis concomitantly with raised intracellular pH (pHi) in quiescent monolayer Chang liver cells. Genistein produces a dose-dependent suppression of ATP-induced DNA synthesis without affecting cell viability (dead cell index ≈ 5%) or pHi elevation. The results suggest that (a) mitogenesis and pHi are not imperative correlates and (b) genistein is effective in inhibiting DNA synthesis whether due to growth factor or “non-growth factor” stimulation.
