Abstract
1) The water content of lotus-seed collected from the bottom-mud of pond was determined by the use of two methods.
2) The apparatus for Karl Fischer's back titration method was modified by the writers, and used in this study.
3) The results obtained from the different regions of seed by these two methods were compared, and then discussed.
4) Because the moisture content of the seed is not extremly small, the reason why the lotus-seed retain the vitality for a long time may not be in the smallness of water content.
Acknowledgment is due to Dr. K. Yusawa for providing facilities and for his helpful suggestions and encouragement, and also to Dr. I. Ohga for his kindness in giving the sample used in this study and for his sincere advice.