Control of nuclear conjugation in the Buller Phenomenon by doubly compatible combinations

Abstract

A study was made on a genetical control of nuclear conjugation in doubly compatible di-mon matings, using Coprinus macrorhizus f. microsporus. By crossing each of different monokaryons with a standard one for five successive generations, isogenic monokaryons in regard to the modifiers which seem to be independent of the incompatibility factors and seem to control nuclear conjugation, were produced. By using these isogenic monokaryons and original ones, two series of experiments were conducted.
First, doubly compatible di-mon matings were made, in each of which one nucleus of the dikaryon was isogenic with the nucleus of the monokaryon and another nucleus of the dikaryon was original. In each of the above matings, the original nucleus of the dikaryon always conjugated with the nucleus of the monokaryon without any exception.
Secondly, doubly compatible di-mon matings were made, using isogenic monokaryons only. The nuclear constitutions of the resulting dikaryotic hyphae were of two kinds in each mating.
From these results, it was revealed that the selective conjugation of nuclei in doubly compatible di-mon matings was caused by the difference of the modifying alleles.