1970 Volume 12 Issue 3 Pages 365-382
In the previous papers of the study it was reported that in the patients of pyelonephritis and in animal 4experiments, the enterobacterial common antigen-antibody system behaves in a different way from that of Jthe enterobacterial O antigen-antibody system. The purpose of the present study is to classify the immunoglobulins in which the common and O antibodies are contained. For this purpose, rabbits were immunized with heated suspension of E. coli 014, ethanol soluble fraction of E. coli 055 and supernate of E. coli 0111 mixed with complete Freund's adjuvant. Experimental pyelonephritis of rabbits was produced by intravenous injection of E. coli 014 and 055 after ligation of unilateral ureter. The sera from the patients and rabbits were fractionated by gel filtration on Sephadex G-200 and the fractions were measured on the common antibody titer by hemagglutination and on the O antibody titer by hemagglutination inhibition reaction.The results were as follows. Enterobacterial common antibody was produced in 19 S globulin in every case. Enterobacterial O antibody was excessively produced in 19 S class of immunoglobulin, but evidence was obtained that small amount of O antibody was also produced in 7 S globulin in the sera obtained from the rabbits of experimental hematogenous pyelonephritis infected with E. coli 055 and the rabbits immunized with supernate of E. coli 0111 mixed with complete Freund's adjuvant. It is suggested that particular feature of enterobacterial common antigen-antibody system is due to the fact that enterobacterial common antibody is produced in 19 S fraction only.
