Abstract
Renal Micropuncture study of the Wistar strain rat was performed and the following results were obtained.Results; (1) In the proximal tubule of the control rat Kidney, [TF/P]inulin was 1.86+0.64, [TF/P]osm 0.97±0.05, [TF/P]Na 0.99+0.03, and [TF/P]K 1.02+0.07, respectively. There were no significant change in [TF/P] values of the above menthioned solutes under the control, diuretic and antidiuretic conditions. (2) In the distal tubule, [TF/P]osm, Na were lower under the antidiuretic condition, whereas [TF/P]Na was only slightly depressed under the diuretic condition. (3) In the samples of ureter fluids, [TF/P]inulin, osm, Na, K were lowered under the diuretic condition, , whereas [TF/P] inuiln, osm, K were elevated but [TF/P] Na was depressed under the antidiuretic condition. (4) Pitfalls of Micropuncture Study (i) Negative pressure of sampling fluids should be avoided, since fluid movenent backwards from distal site made significant errors. (ii) In order to avoid contamination of inulin estimation, accurate sampling into the microcuvette and complete removal of air in cuvette were needed. (iii) Amount of samples should be same as far as possible for the osmorality determination.
