1974 Volume 16 Issue 4 Pages 379-389
Guanidine base was determined using activated charcoal according to Yatzidis, and methylguanidine was separated by ion exchange columnchromatography(Dowe×50), and then measured colorimetrically by the Sakaguchi reaction. By using these technigues, serum levels and urinary excretions of guanidino derivatives in uremic and control subjects were compared. 1) Serum level of guanidine base in 11 normal subjects was 0.3±0.1 mg/dl, and 1.5±0.4 mg/dl in 28 uremic subjects. 2) Serum level of methylguanidine in 6 normal subjects was 0.0046±0.002 mg/dl, and 0.093±0.043 mg/dl in 19 uremic subjects. 3) Urinary excretion of methylguanidine was 0.5±0.35mg/day in normal subjects, and 10.0±0.43 mg/day in uremic subjects. 4) A correlation between guandine base and serum creatinine (r=0.88) was more closer than the blood nitrogen (r=0.63). 5) The guanidino derivatives were dialyzable easily by cuprophane membrane, and the value of guanidine and creatinine dialyzance was the same in the standard kiil dialyzer.
