Studies on the Adenine Nucleotides Metabolism in Uremic Erythrocyte

Abstract

The metabolic disorders in the erythrocyte of the patients with chronic renal failure were investigated in the aspect of adenine nucleotides metabolism, and the following results were ob-tained. 1) ATP content of uremic erythrocyte was increased. 2) Biosynthesis of ATP from adenine was activated in uremic erythrocyte, and this was con-sidered to be caused by a high ATP level in uremic erythrocyte. 3) Both intra- and extra-cellular accelerating factors which activated biosynthesis of ATP in uremic erythrocyte were demonstrated. 4) These factors could not be dializable by the procedure of regular hemodialysis. 5) Extra-cellular factor was mainly detected in the low molecular weight fraction (less than M.W.10, 000) in uremia serum, and this factor was impossible to identify with so-called uremia toxins, 6) Intra-cellular factor was also detected in the low molecular weight fraction (less than M.W. 10, 000) in uremia erythrocyte, and this factor was heat stable and was not soluble in the Folch's solution. These obtained results indicate the limit of the present hemodialysis, and receuire the develo-pment of new treatment based on the view point of biochemical disorder for chronic renal failure.