Guanidino compounds in various tissues of the rats with chronic or acute renal failure

Abstract

Experiments were performed on rats with chronic or acute renal failure in order to investigate the alteration of guanidino compounds. The purpose of this paper is to gain the profiles of urea cycle metabolism in renal failure, because the guanidino compounds, as uremic toxins, would take an important part in elucidating the clinical features of uremia. Chronic renal failure was produced by the method of Platt and acute renal failure was produced by bilateral Iigation of the ureter in male roister rats. The concentration of guanidino compounds in the plasma, brain, liver, kidney, skeletal muscle and intestine were measured by JASCO G-520 guanidine autoanalyser. In the rats with chronic renal failure, the concentration of guanidinosuccinic acid(GSA), guanidinobutyric acid (GPA), guanidine (G) and methylguanidine (MG) were increased in almost every organs, while arginine (Arg.) remained unchanged. However, the concentration of all guanidino compounds were not changed in the brain. In the rats with acute renal failure, a decrease of guanidinoacetic acid (GAA) and an increase of GSA, G and MG were detected in each organs. Furthermore, there was a decrease of Arg, and an increase of G and MG in the brain.