IgA腎症の免疫学的検討 ―T cell機能および単球と血中immune complexとの関連性について―

大園 恵幸

doi:10.14842/jpnjnephrol1959.29.961

Immunological study of IgA nephropathy

-Relationship between T cell function, monocyte and circulating immune complex-

YOSHIYUKI OHZONO

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Keywords: IgA nephropathy, T cell, monocyte, circulating immune complex, gel filtration

JOURNAL FREE ACCESS

1987 Volume 29 Issue 8 Pages 961-972

DOI https://doi.org/10.14842/jpnjnephrol1959.29.961

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Published: August 25, 1987 Received: February 25, 1987 Available on J-STAGE: July 05, 2010 Accepted: - Advance online publication: - Revised: -
Correction information
Date of correction: July 05, 2010 Reason for correction: - Correction: DTRECEIVED Details: Right : 19870225

Date of correction: July 05, 2010 Reason for correction: - Correction: SUBTITLE Details: [in Japanese]

Date of correction: July 05, 2010 Reason for correction: - Correction: CITATION Details: Wrong : 1) Berger, J.: IgA glomerular deposit in renal disease, Transplant. Proc. 1 : 939-944, 1969.
2) Sakai, H., Nomoto, Y. & Arimori, : Deo crease of IgA specific suppressor T cell activity in patients with IgA nephropathy. Clin, exp. Immunol., 38 : 243-248, 1979.
3) Egido, J., Blasco, J., Sancho, J. & Lozano, L.: T cell Dysfunctions in IgA nephropathy : Specific abnormalities in the Regulation of IgA synthesis. Cling Immunol., 26 : 201-212, 1983.
4) Woodroffe, A.J., Gormaly, A.A., Seymow, A.E. & Clarkson, A. R.: Immunologic studies in IgA nephropathy. Kidney. Int. 18 : 366-374, 1980.
5) Stauchwa, I., Singb, G., Whiteside, T. L.: Im-muno abnormalities in IgA nephropathy (Berger's disease). Clin. Immunopath. 20 : 379-388, 1981.
6) Lawrence, S., Pussell, B. A. & Clarkes worth, J. A.: Mesangial IgA nephropathy : detection defective reticulo phagocytic function in vivo. Clin. Nephrol., 16 : 280-283, 1983.
7) Ooi, Y. M., Ooi, B. S.: Identification of mono cyte phagocytic defect in subpopulation of pati-ents with nephritis. Kidney Int., 23 : 851-854, 1983.
10) Voller, A., Bridwell, D. E. & Bartlett, A.: The enzyme linked immune sorbent assay (ELISA). A review with a bibliography of microplate ap-plications Flowing publications, 1-48, 1977.
11) Perira, A. B., Theofilopoulous, A. N. & Dixone, F. T.: Detection & partial characterization of circulating immune complexes with solid phase anti C3. T. Clin. Immunol., 125 : 736-777, 1980.
13) Yonemasu, K. & Stroud, R. M.: Clq rapid purfication method for preparation of monospecific anti sera and for biochemical studies. T. Im-munol. 106 : 304-313, 1971.
14) Ledbetter, J. A., Evans, R. L., Lipinski, M., Cunningham, R. C., Good, R. A. & Herzenberg, L. A.: Evolution Conservation of Surface Mole-cules that distinguish T lymphocyte helper/inducer and T cytotoxic/Suppressor subpopula-tions in Mouse and Man. J. Exp, med., 153 : 310-323, 1981.
15) Egido, T., Biasco, R., Sancho, J., Iescs, M. & Hernandes, L.: Abnormalities of immune regula- Lion in patients with IgA mesangial glomerulo-nephritis (Berger's disease), Proc. EDTA., 19 : 642-647, 1982.
16) Nomoto, Y., Sakai, H. & Arimori, S.: Increase of IgA bearing lymphocyte in peripheral blood from patients IgA nephropathy. Am. J. Clin. Pathol,, 71 : 158-160, 1979.
17) Sakai, H., Nomoto, Y., Arimori, S., Komori, K., Inoue, H. & Tsuji, K.: Increase of IgA bearing peripheral blood lymphocytes in families of patients with IgA nephropathy. Am. J. Clin. Pathol., 72 : 452, 1972.
18) Cosio, F. G., Lem, S., Folani, A. O., Conley, M. E. & Michael, A. F.: Immune regulation of immunoglobulin production in IgA nephropathy. Clin, Immunol, and Immunopathol., 23 : 430-436, 1982.
19) Egido, T., Blasco, R., Sancho, J., Lozxano, L., Sanchez-Crespon & Hernando, L.: Increase rates of polymeric IgA synthesis by circulating lym-phoid cells in IgA mesangial glomerulonephritis. 47 : 309-316, 1982.
20) Lopez, T. M., Egido, J., Sancho, J. & Hernande, L.: IgA glomerulonephritis (Berger's disease). Evidence of high serum lebels of polymeric IgA. Clin. exp. Immunol., 42 : 247, 1980.
21) Coppo, R., Basolo, B., Martina, G., Rollino, C., Marchi, M., Giachino, F., Mazzucco, G., Messina, M. & Picoli, G.: IgA nephropathy and in Scholein Henoch nephritis. Cornela Correlation with clinical and histological signs of activity. Clin. Nephrol., 18 : 230-239, 1982.
22) Berger, T., Yaneva, H., Nabarra, B. & Bar-banel, C.: Recurrence of mesangial deposition of IgA after renal transplantation. Kidney Int.,7 : 232, 1975.
23) Gemuth, F. G. Jr., Senterfit, L. B. & Dreesman, G. R.: Immune complex disease. V. The nature of the circulating complexes associated with glo-merular alteration in the chronic BSA rabbit system. Hopkins Med. J., 130 : 344-357, 1971.
24) Roccatello, D., Coppo, R., Basolo, B., Mathina, G., Rollino, C., Cordonnier, D., Busqet, D., Ric-ciotlo, G., Sena, L. M. & Diccoli, G.: Interaction between the macrophage system and IgA immune complex in IgA nephropathy. Pro. EDTA., 20 : 610-616, 1983.
25) Guido Monga, M. D., Gianna Mazzucc, M. D., Giovonni Barbiano C.I., Belgiozoso, M. D. & Chil Busnach, M. D.: Monocyte Infiltration and Glo-merular Hypercellurality in Human Acute and Persistent Glomerulonephritis.: Light and Elec-tron Microscopic, Immuno fluorescence, and His-tochemical Investigation on Twenty Eight Cases Lab. Invest, 44 : 381-387, 1981.
26) Alex, B., Magil, M. D., Louis, D. & Wadsworth, M. B.: Monocytes in Human Glomerulonephritis. An Electron Microscopic Study. Lab., Investi., 45 : 77-81, 1981.
27) Berthoux, F. C., Gazne, A., Sabatien, T. C., Ducret, F., Lepetit, J. C., Marcellin, M., Mercier, B. & Brizard, C. P.: HLA-BW 35, mesangial IgA glomerulonephritis. N. Eng. J. Med., 298 : 1034-1035, 1978.
28) Richman, A. V., Mahoney, J. T. & Fuller, T.J.: Higher prevalence of HLA-B-12 in patients with IgA nephropathy. Ann. Intern. Med., 90 : 201, 1979.
29) Kashiwabara, K., Schishido, H., Tomura, S.,Tuchida, H. & Miyajima, T.: Strong association between IgA nephropathy & HLA-DR 4 antigen. Kidney Int., 22 : 377-382, 1982.

Right : 1) Berger, J.: IgA glomerular deposit in renal disease, Transplant. Proc. 1 : 939-944, 1969.
2) Sakai, H., Nomoto, Y. & Arimori, : Deo crease of IgA specific suppressor T cell activity in patients with IgA nephropathy. Clin, exp. Immunol., 38 : 243-248, 1979.
3) Egido, J., Blasco, J., Sancho, J. & Lozano, L.: T cell Dysfunctions in IgA nephropathy : Specific abnormalities in the Regulation of IgA synthesis. Cling Immunol., 26 : 201-212, 1983.
4) Woodroffe, A.J., Gormaly, A.A., Seymow, A.E. & Clarkson, A. R.: Immunologic studies in IgA nephropathy. Kidney. Int. 18 : 366-374, 1980.
5) Stauchwa, I., Singb, G., Whiteside, T. L.: Im-muno abnormalities in IgA nephropathy (Berger's disease). Clin. Immunopath. 20 : 379-388, 1981.
6) Lawrence, S., Pussell, B. A. & Clarkes worth, J. A.: Mesangial IgA nephropathy : detection defective reticulo phagocytic function in vivo. Clin. Nephrol., 16 : 280-283, 1983.
7) Ooi, Y. M., Ooi, B. S.: Identification of mono cyte phagocytic defect in subpopulation of pati-ents with nephritis. Kidney Int., 23 : 851-854, 1983.
10) Voller, A., Bridwell, D. E. & Bartlett, A.: The enzyme linked immune sorbent assay (ELISA). A review with a bibliography of microplate ap-plications Flowing publications, 1-48, 1977.
11) Perira, A. B., Theofilopoulous, A. N. & Dixone, F. T.: Detection & partial characterization of circulating immune complexes with solid phase anti C3. T. Clin. Immunol., 125 : 736-777, 1980.
13) Yonemasu, K. & Stroud, R. M.: Clq rapid purfication method for preparation of monospecific anti sera and for biochemical studies. T. Im-munol. 106 : 304-313, 1971.
14) Ledbetter, J. A., Evans, R. L., Lipinski, M., Cunningham, R. C., Good, R. A. & Herzenberg, L. A.: Evolution Conservation of Surface Mole-cules that distinguish T lymphocyte helper/inducer and T cytotoxic/Suppressor subpopula-tions in Mouse and Man. J. Exp, med., 153 : 310-323, 1981.
15) Egido, T., Biasco, R., Sancho, J., Iescs, M. & Hernandes, L.: Abnormalities of immune regula- Lion in patients with IgA mesangial glomerulo-nephritis (Berger's disease), Proc. EDTA., 19 : 642-647, 1982.
16) Nomoto, Y., Sakai, H. & Arimori, S.: Increase of IgA bearing lymphocyte in peripheral blood from patients IgA nephropathy. Am. J. Clin. Pathol,, 71 : 158-160, 1979.
17) Sakai, H., Nomoto, Y., Arimori, S., Komori, K., Inoue, H. & Tsuji, K.: Increase of IgA bearing peripheral blood lymphocytes in families of patients with IgA nephropathy. Am. J. Clin. Pathol., 72 : 452, 1972.
18) Cosio, F. G., Lem, S., Folani, A. O., Conley, M. E. & Michael, A. F.: Immune regulation of immunoglobulin production in IgA nephropathy. Clin, Immunol, and Immunopathol., 23 : 430-436, 1982.
19) Egido, T., Blasco, R., Sancho, J., Lozxano, L., Sanchez-Crespon & Hernando, L.: Increase rates of polymeric IgA synthesis by circulating lym-phoid cells in IgA mesangial glomerulonephritis. 47 : 309-316, 1982.
20) Lopez, T. M., Egido, J., Sancho, J. & Hernande, L.: IgA glomerulonephritis (Berger's disease). Evidence of high serum lebels of polymeric IgA. Clin. exp. Immunol., 42 : 247, 1980.
21) Coppo, R., Basolo, B., Martina, G., Rollino, C., Marchi, M., Giachino, F., Mazzucco, G., Messina, M. & Picoli, G.: IgA nephropathy and in Scholein Henoch nephritis. Cornela Correlation with clinical and histological signs of activity. Clin. Nephrol., 18 : 230-239, 1982.
22) Berger, T., Yaneva, H., Nabarra, B. & Bar-banel, C.: Recurrence of mesangial deposition of IgA after renal transplantation. Kidney Int.,7 : 232, 1975.
23) Gemuth, F. G. Jr., Senterfit, L. B. & Dreesman, G. R.: Immune complex disease. V. The nature of the circulating complexes associated with glo-merular alteration in the chronic BSA rabbit system. Hopkins Med. J., 130 : 344-357, 1971.
24) Roccatello, D., Coppo, R., Basolo, B., Mathina, G., Rollino, C., Cordonnier, D., Busqet, D., Ric-ciotlo, G., Sena, L. M. & Diccoli, G.: Interaction between the macrophage system and IgA immune complex in IgA nephropathy. Pro. EDTA., 20 : 610-616, 1983.
25) Guido Monga, M. D., Gianna Mazzucc, M. D., Giovonni Barbiano C.I., Belgiozoso, M. D. & Chil Busnach, M. D.: Monocyte Infiltration and Glo-merular Hypercellurality in Human Acute and Persistent Glomerulonephritis.: Light and Elec-tron Microscopic, Immuno fluorescence, and His-tochemical Investigation on Twenty Eight Cases Lab. Invest, 44 : 381-387, 1981.
26) Alex, B., Magil, M. D., Louis, D. & Wadsworth, M. B.: Monocytes in Human Glomerulonephritis. An Electron Microscopic Study. Lab., Investi., 45 : 77-81, 1981.
27) Berthoux, F. C., Gazne, A., Sabatien, T. C., Ducret, F., Lepetit, J. C., Marcellin, M., Mercier, B. & Brizard, C. P.: HLA-BW 35, mesangial IgA glomerulonephritis. N. Eng. J. Med., 298 : 1034-1035, 1978.
28) Richman, A. V., Mahoney, J. T. & Fuller, T.J.: Higher prevalence of HLA-B-12 in patients with IgA nephropathy. Ann. Intern. Med., 90 : 201, 1979.
29) Kashiwabara, K., Schishido, H., Tomura, S.,Tuchida, H. & Miyajima, T.: Strong association between IgA nephropathy & HLA-DR 4 antigen. Kidney Int., 22 : 377-382, 1982.

Abstract

In order to verify whether cell mediated immunity and circulating immune complexes play a role in the pathogenesis of IgA nephropathy, cell mediated immunity was studied in T cell, B cell subsets, T cell function and the influence of monocytes on immuno-globulin production in patients with IgA nephropathy. CIC were measured by using Clq EIA and anti C3 EIA. Relation between CIC and T cells and monocytes were also studied. The results were followed as. 1) The percentage of Leu 2a cells in peripheral lymphocytes was significantly lower and that of Leu 3a cells higher in patients with IgA nephropathy compared with other groups, meaning that an elevated Leu 3a/Leu 2a cell ratio was observed in patients with IgA nephropathy. 2) IgA T cell helper activity was significantly elevated and IgG and IgM T cell helper activity were also slightly elevated in patients with IgA nephropathy. 3) The higher incidence of IgA immune complex was observed in patients with IgA nephropathy compared with other groups. 4) IgA T cell helper activity in patients with positive IgA-IC was significantly more elevated than in patients with negative IgA-IC. 5) The percentage of monocytes which IgA granular deposits were detected in the cytoplasma or on the cell surface in patients with IgA nephropathy was signficantly higer than in healthy subjects. 6) An enhancement of IgA production was observed when monocytes from patients with IgA nephropathy were added into pokeweed mitogen stimulated coculure with T cells and B cells from healthy subjects. 7) An enhancement of IgA production by T cells treated with IgA-IC was observed compared with T cells treated with IgG-IC or IgM-IC, 8) An enhancement of IgA production by monocytes treated with IgA-IC from IgA nephropathy patients was revealed compared with monocytes treated with IgG-IC or IgM-IC.

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