Pure culture of the tubercle bacillus in the urogenitaltuberculose

Abstract

I carried cut the pure culture of tubercle bacillus in the urogenital tuberculosis; namely 131 cases in mines of renaltuberculosls; one case in the pus of renal tuberculesis, 3 cases in tuber culousepidydimitis.
1) I preferred Petroff's, Lownstein- 8umiycshi's, and Hohns' methods as cultural methods and used Petroff's, glycerinepotato, potato-egg, Hohns, and Saponin-egg as media.
2) By Petroff's methods 34 cases were proved possitive in 35 microscopically possitive cases, and 16 cases were proved possitive in 20 microscopically negativeones
3) Tubercle bacillus in glycerine-potato less grown than in Petroff's medium, and treatment with sod. hydrate solution was less efficient than with sulphuric acid.
4) By my researches in Hohn's methods 33 cases were proved possitive in 34 microscopically possilive cases (with exceptions of 2 cases which proved unsuccesful by animal and cultural experiments) and 20 cases were proved possitive in 36 microscopically negative ones.
5) The results of experiments carried out by various media by Hohns' methods were as follow: -in Saponin- egg 12 cases (75%), in Hohns' 50 ones (74%), in Petroffs' 16 cases (59%) and in potato-egg 16 (59%) proved passitive.
6) By Hohn's methods the minimum days, in which colonies were found macroscopically, were 8day's in cases of Saponin-egg; 12 days, Hohns' 13days, potato-egg; 17days, Petroff's medium; while maximum and average days are as referred to table IX.
7) Petroff's and Löwensteui-Sumiyoshi's were rather complicated than that of Hohn's methods and were apt to be contaminated and chavasteristic merit of far greator simplieity and efficiency of operation by Hohn's also recognizable, and days to be refuired for developement of ealony were found shorter than by any other methods.
8) Two cases of urines which were tubercle bacilli microscopically possitive, proved negative by animal and cultural test, while one cases of urines which was negative under microscopically and cultural tests brought about tuberculous change by animal.
9) The cultural tubes are desirable to use ten glasses for each case at least.
10) I recognized that tubercle bacillus is comptely sterilied by the treatinent of 10% solution of HNO 3.
11) Hrom the experimental results mentioned above I con-inced culture is far superior to microscopical investigation and culture is not superior to animaltest, but is almost of the same value as compared with it, and it is most scrupulous to apply both methds as described above and the great charasteristic of the culture is that by which an avain type is easily obtained. Culture of the bacillus must be most necdful when tubercle bacillus is negative through microscope with suspision of existence of tuberculosis from clinical diagnosis, especially in the urine of tuberculous kidney.