Colloid behavior of urine

Abstract

On the colloid behavior of urine much has been written, but little decided. Especially in our urological sphere it is lacking in recognition of its behavior and wanting in experimental data. To measure the amount of colloid in urine there are three methods, by measuring the surface tension, viscosity and the protective action of emulsoid in urine to the precipitation of suspensoid by crystalloid, The former two methods have many experimental factors, so this author has measured by the third, such as the socalled “Goldsol reaction” and the “Gold number” by Zsigmondy.
The goldsol is prepared by the Narabayashi's method, and 0.4% and 2.0% salt solution are employed for the dilution of urine. By former 0.4% salt solution this author find the precipitating curve by crystalloid of urine and by 2.0% salt solution the protective curve of colloid in urine. The urine is diluted four times by these solution. In each test tube 1.0ccm of diluted urine and 5.0ccm of gold sol are added and shakeed for a few minutes. After a quarter of an hour the change of the color in test tubes will be observed. This is the brief description of the authors procedure to determine the gold sol reaction for urine. To measure the gold number the original method of Zsigmondy is employed, but is rather different in its expression. This number is shown in ccm of dialyzed urine. The results of the experiment are as follows:
1) In normal cases the urine shows similar precipitating and protective curve for gold sol. The precipitating action will be held chiefly by the electrolytes in urine and the protective colloid in urine.
2) In nervous cases the gold sol reaction of urine compares well with that of normal cases.
3) In urological inflammatory cases especially in cystitis the amount of protective colloid is increased remarkably point formation.
4) In a few cases the protective curve shows the “Zackenbildung”.
5) The precipitating and protective colloid will be distinguished in the colloid of urine.
6) The precipitating action of urine is concerns the specific gravity of urine.
7) The gold number in normal cases is from 0.8 to 2.0. The average number is 1.4.
8) The gold number in lithiasis is from 0.1 to 2.3. The average number is 0.8. Thus such small number in lithiasis has close relation to the complication of this disease.
9) The precipitating action of urine in lithiasis bears resemblance to normal urine, but the protective action is larger than the normal action of urine.
10) The gold sol reaction of urine in postlithiasis is changed by complication.
11) The gold number of urine in lithiasis has always no close relation to its protective action.
12) By fever the amount of colloid in urine is increased.
13) The mucin in saliva shows protective action in the normal urine.
14) In normal and nervous cases this author can not find out the “acetic acid body” in urine. But in some urological inflamatory cases this is obsevred in urine. The “acetic acid body” is considered to have some relation to haemorrhage in the urinary tract.