1952 Volume 43 Issue 4 Pages 146-172
This is a part of the study pertaining to the absorption of therapeutic salts through the lower urinal tract, which has being conducted by Prof. Tabayashi and done at the Department of Urology, Tokyo Medical College.
A study on the absorption and penetration of the therapeutic silver salt in the urethra (anterior and posterior) and in the bladder has previously been published (by Aika and Abe) in detail in Sections I, II and III of these studies. However, these studies have been confined chiefly to histological examinations, the amount of absorption has not been calculated. Moreover, with respect to the absorbility of silver drugs, such as Protargol, which practically do not react to any silver reaction of the tissue, was neglected as the object of this experiment.
I have calculated the amount of the above mentioned silver solutions absorbed by the bladder, and also the amount of its movement to the other organ exclusively by means of biochemical methods. These were done by injecting 30cc of 0.1% argen. nit. solution and 3% protargol solution into the rabbit's bladder. These were calculated at eight intervals of 15 minutes, 30 minutes, 1-2-4-6-12 and 24 hours.
To meet this process, the silver amount remaining in the bladder was calculated by the chemical method conformable to each of the solutions (silver nitrate and protargol), and the amount absorbed was counted up and compared with the amount of silver contained in the above mentioned solution. At the same time, the distribution of silver within the inner organs such as bladder, liver, kidney, etc. was verified by this experimentation. These calculations were made by weight after calcination.
The details are shown in Tables 1 and 9 with the chart of various curves and photos. The actual amount of silver absorbed from these two kinds of solution and its percentage at each minute and hour are as follows:
1. Argentum nitricum solution 0.1% 30cc Injection
2. Argentum protenicum (Protargol) 3% 30cc Injection
According to these figures the absorption of silver nitrate was about 40 at 30 minutes after the injection, but the absorption does not progress further on in proportion of the lapse of time. That is to say, the amount after 24 hours was not more than 41.7%. In other words, the sbsorption of silver nitrate solution has not taken place in proportion to the time, but within the first 30 minutes after the onset of injection most part of the silver has been sbsorbed. And between 30 minutes and 12 hours the curve of the amount absorbed showed an undulation. Between 12 and 24 hours the amount absorbed was not more than 1%.
The maximum amount of the absorption of protein silver in the beginning, reaching at its peak of 2 hours after injection, was later than of silver nitrate. Its rate was less (27.9%) than that of silver nitrate. (However, this protein silver has a high density of 3%, so the actual amount to be absorbed must be far greater than that of silver nitrate). The absorption did not advance between 2 to 12 hours, but after 12 to 24 hours after the injection the absorption has again taken place. While the 50% of protein silver is absorbed after 24 hours, this is the different point from the absorption of silver nitrate.
It can be demonstrated that these two kinds of silver particles absorbed through the bladder, can be examined by calcinating the bladder, liver, kidney and other tissues.
Generally, in these silver therapeutic solutions, the actual amount of silver absorbed and the absorption of the solvent do not develop in pararell, but both silver and solvent respectively indicate their each different absorption curve.
Moreover, a similar experiment was conducted, without isolating the bladder by operation, but merely injecting the above silver solution in the bladder and closing the mouth of the urethra. The result obtained, as for protein silver, was practically the same.
As to silver nitrate, the amount abosorbed after injection was
