SPECTROANALYTIC STUDIES ON PENICILLIN G

Abstract

1) Ultraviolet absorption spectra of the penicillin G and its related substances have been studied, Characteristic absorption band with penicillin G aq. solution lies between 260mμ and 270mμ which may be correlated with its chemotherapeutical active portion of the molecule.
2) By measuring the optical density at 265mμ of the penicillin G solution whose concentration is unknown, its international units can be calculated by means of the following two equations.
a=372×0.9/210logI₀/I (a: mg/cc)
u=1667a (u: international units per cc)