1957 Volume 48 Issue 7 Pages 524-533
The emulsion of human tuberculous kidney, from which protein had been removed by boiling, was injected intravenously in normal male rabbits for three weeks. The body weight, total serum protein, HST (Hepatsulpharen test), and uric Millon's reaction were then examined (Group A). As the controls, non tuberculous renal emulsion (of calculus kianey) (group B) and 0.5% carbol physiolgical saline solution (group C) were injected in other group of rabbits. Afterwards the rabbits were killed by air embolus, and the liver, kidney, spleen, lung, and heart of the animals were examined histologically and histochemically.
1) In any rabbit of group A, B and C, the body weight and total serum protein dosis did not show any definite change.
2) In rabbits injected with tuberculous kidney emulsion (group A), a significant increase in HST was observed (α=0.05) after 3 weeks. In rabbits injected with normal kidney emulsion (group B), the HST showed an increasing tendency, though the increase after 3 weeks was not definite. In rabbits injected with phisiologic saline solution, the HST showed no essential change.
3) A change in the uric Millon's reaction was almost similar to that of HST. In the cases of group A, injected with tuberculous kidney emulsion, the uric Millon's reaction increased remarkably. After 3 weeks a significant increase of the uric Millon's reaction was observed. In some cases in group B, injected with normal kidney emulsion, the uric Millon's reaction increased. However, an increase of average value was not significant. In group C, the Millon's reaction showed no essential change.
4) In the liver of group A rabbits, a degenerative atrophy of liver cells, bleeding, necrosis, fat degeneration, disappearance or remarkable decrease of PAS reaction positive substances, and decrease of alkaline phosphatase reaction were observed remarkably. However, in the liver of group B rabbits, these change were not remarkable. In group C, these histological and histochemical changes were hardly observed. In rabbits in which uric Millon's reaction showed a very high value, these degenerative changes of the liver were more remarkable than the others.
5) The histological and histochemical changes of the kidney in all rabbits in group A, B and C, were not remakable. In the spleen, lung, and heart of these rabbits, the histological and histochemical changes were hardly observable.
6) It is concluded that an increase in uric Millon's reaction may cause degenerative changes of the liver as described above. Except for the patients complicated with a serious distarbance of total renal function, therefore, the uric Millon's reaction may be a reliable means of testing the liver function.
