STUDIES ON INTESTINAL GAS THAT HINDERS ROENTGEN DIAGNOSIS

PART II EXPERIMENTAL STUDIES

Abstract

We discussed the problem on intestinal gas in Part I. At that time we explained that this problem was complicated, but that it would be possible to remove intestinal gas by some method to such an extent as not to hinder roentgen diagnosis. Though intestinal gas can be classified into swallowed air and gas produced by intestinal flora, it is possible to remove intestinal gas by effectuating nonactivation of the intestinal flora.
Faces from the 8 patients who were described in Part I were divided into two parts. One part was used as a source of isolation and 8 strains of Escherichia coli were prepared by isolation and culture according to the conventional technique. Regarding the other part, 5g. of it was dissolved in 5cc of sterilized physiological salt solution and 8 kinds of mucus feces solutions were prepared. Part II dealt with the results of the below-mentioned examinations.
As basal media a 1% glucose-peptone solution and a 1% glucose-bouillon were prepared using the Smith fermentation tube. The definite amounts of the aseptically-treated 7 drugs mentioned in Part I were suspended in these basal media and two sets of media consisting of 7 each were thus prepared. The 8 strains of colon bacillus and definite quantities of the smeary feces solution were implanted into each of these media, and determination was made of the amounts of gas produced for 24 hours. As a control the basal media were used with those to which the drugs were added. Irrespective of the colon bacillus and smeary solution, homosulfamine and streptomycin inhibited gas production in this order. In contrast to this, restamine gave riso to extraordinarily increased gas production.