A STUDY ON THE CULTURE OF GONOCOCCUS IN FLUID MEDIA

Abstract

The study was performed on conditions and media components of the fluid cultures of neisseria gonorrhoeae. Additionally it was confirmed that determination of growth turbidity with the electrophotometer was considerably accurate.
After diffuse resuspending of bacteria in normal saline by vigorous shaking, the determination of turbidity with the electric photometer (550mμ) revealed enough growth grade of the bacteria, regardless of the kind of Strains or cultivation time. It also indicated indirectly the wet weight or N. dose of bacteria.
In regard to the components of culture media, proteose peptone No. 3 was effective as a Base substance, but meat infusion or meat extract showed a few inhibitions for gonococcus growth. The optical density of each component is: of proteose peptone No. 32%, glucose 0.2%, Sodium Chloride 0.5%, soluble starch 0.1%, but yeast extract and phytone in autoclaved media were variable. L-cystine HCl at 0.1‰ effected inhibitive or a mounting action.
As to the cultural condition, one of the shallow and wide surface media was most suitable, increased CO₂ was also effective on bacteria growth. Otherwise “Shaking culture” and glass beads in fluid media by yielded almost no augmenting on bacteria growth.
From the experimental results, the comperative studies on the same doses of fluid media and 10% horse blood-proteose No. 3 agar were made. It was revealed that fluid media were superior from the viewpoint of the harvest, but the growth velocity of bacteria on these media is smaller.