DESOXYRIBONUCLEIC ACID (DNA) CONTENT IN THE EXPERIMENTALLY INDUCED TUMOR OF THE URINARY BLADDER

Abstract

1) A 0.6g. paraffin pellet containing 100mg. 20-methylcholanthrene which was left in urinary bladder for ten months in 15 rabbits gave rise to bladder papillomas in ten (66.7%).
2) Relative amount of desoxyribonucleic acid (DNA) of the nucleus of epithelial cells was measured in the normal bladders of rabbits by means of microspectrophotometer with Feulgen staining.
The results are as follows:
a) DNA content of the nucleus of epithelial cells at the site of papillary growth was twice to four times as high as normal.
b) DNA content of the nucleus of epithelial cells was normal in the normal-appearing distant area and downgrowth area of the tumor-bearing bladder.