THERMOSENSITIVE DRUG RESISTANCE FACTOR II.
THERMOSENSITIVE ELIMINATION OF THE KANAMYCINRESISTANCE FACTOR FROM THE HOST BACTERIAL CELLS
1967 Volume 58 Issue 1 Pages 9-16
Details
1967 Volume 58 Issue 1 Pages 9-16
A kind of R factor carrying only kanamycin (KM)-resistance marker and originated from a wild strain of Proteus vulgaris was spontaneously eliminated from the host bacterial cells when P. vulgaris (R:KM) was cultured at 420°C, although no elimination occured in the same bacterial cells grown at 25°C.
This thermosensitive elimination of the R(KM) factor was also observed when this R(KM) factor infected the cells of E. coli and Salmonella typhirnurium.
On the other hand, the oridinary drug-resistance factor could not be eliminated by the cultivation at higher temperatures, even in the same host bacterial cell.
From the obtained results, it was considered that this thermosensitive elimination of the R(KM) factor depends upon thermosensitive replication of this R factor.
