A STUDY ON SO-CALLED PROSTATIC EOSINOPHILIA

REPORT 7. CYTOCHEMICAL INVESTIGATION OF CHARCOT-LEYDEN CRYSTALS-FORMING EOSINOCYTES AND THE CRYSTALS

Abstract

Previously the author and his coworker reported the presence of Charcot-Leyden crystals with hexagonal pyramidal shapes in peripheral eosinocytes from the typical form of so-called prostatic eosinophilia. Perhaps the crystals would be assumed to be Charcot-Leyden crystals. This time, cytochemical examination was made of Charcot-Leyden crystals-forming eosinocytes and the Charcot-Leyden crystals. The difference between normal and pathological eosinocytes were studied and thus evidence was obtained to show that the formed crystals could be identified as Charcot-Leyden crystals. This paper deals with the results thus obtained.
Cases and Method
The subjects were the same that were mentioned in a previous report. Thirty-nine male patients with urosis and 4 female patients with uross made up to subject.
Method
Blood was obtained twice on the fassing stomach early in the morning and 2 hours after the meal. The specimen was treated in the same manner as in the previous paper in an effort to count eosinocytes and examine blood pictures. The method f forming Charcot-Leyden crystals was quite the same as reported before, Cytochemical investigation consisted of the peroxidase reaction, the silver reaction, Millon's reaction, the azo reaction, and the chrome reaction. Charcot-Leyden crystals were observed by the peroxidase reaction, Millon's reaction, Giemsa staining, Sudan III staining and toluidine blue staining.
Results
Eosinocytes from so-called prostatic eosinophilia differ qualitatively from normal eosinocytes. Eosinophilic granules from this disease contain tyrosine and gave positive Millon's azo and chrome reactions. These eosinocytes produced Charcot-Leyden crystals, which were substrate proteins composed mainly of tyrosine having nothing to do with any enzyme.