MONOLAYER CULTURE OF HUMAN URINARY BLADDER TUMORS. PART I
1968 Volume 59 Issue 2 Pages 128-134
Details
1968 Volume 59 Issue 2 Pages 128-134
1. Thirty-four human urinary bladder tumors obtained by surgery were grown in tissue culture.
2. Eighteen cases attached on the surface of glass flasks and of eleven cases formed monolayer cell sheet over four weeks.
3. Nine out of the eleven cases formed cell sheet were identified as of pure epithelial origin from the morphological observation.
4. Two (25%) out of eight cases of benign papillomas and grade II of papillary carcinomas, which had been determined histopathologically, attached on the surface of glass flasks and only one (12.5%) of them formed cell sheets. On the other hand, in eight of more malignant cases, grade III of transitional cell carcinomas, five were successful in attachment (63%) and four (50%) formed cell sheet, while in seven cases of grade IV of transitional cell carcinomas five were successful in attachment (70%) and three (43%) formed cell sheet.
5. It would be concluded that more malignant carcinomas of the urinary bladder were more successful in monolayer culture.
6. A case from grade III of transitional cell carcinoma formed epithelial cell sheets, which has been continued to grow and multiply over 12 months passing over 8 times of transfers.
This is the first report on a successful long term monolayer culture originated from carcinoma of human urinary bladder.
