ORGAN CULTUTRE ON TUMOR OF THE URINARY BLADDER
(1) A STUDY ON THE METHOD OF ORGAN CULTURE UNDER HYPERBARIC OXYGENATION
1968 Volume 59 Issue 5 Pages 425-433
Details
1968 Volume 59 Issue 5 Pages 425-433
A method of organ culture under hyperbaric oxygenation using the closed chamber system was utilized for the present study on both papillary and invasive tumors and normal mucosa of human urinary bladder. Those tissues were fragmented approximately 0.2cm in diameter and placed on a piece of silicon pause then cultured in the petri dishes with the fluid medium containing 10 per cent calf serum added to Eagle's medium. A fragment of gelfoam sponge placed on the bottom of each culture dish was used as a raft.
The cultures were maintained in an incubator at 37C of temperature for 5 days under various gas phases, which were pure air, air containing 40 per cent oxygen and 100 per cent oxygen and also under one and two atmospheres on each. The pH of the culture media was controlled at 7.2 to 7.4 with the difusion of carbon dioxide gas into the gas chambers according to the color indication of each culture medium.
Satisfactory results were obtained in the culture groups of papillary tumors which were maintained in pure air at 2 atmospheres and in air containing 40 per cent of oxygen at 1 atmosphere. As to the invasive type of carcinomas, however, it was more difficult to preserve the structure in this system; i. e. the satisfactory results were obtained in about a half of the cultures maintained in the same gas phases. Normal mucosa of the urinary bladder was easily affected by the minor deviation of oxygen contents and alter the original cellular characteristics.
Some histochemical studies on the cultures suggested a corelation between the degree of reaction of LDH and histological findings on the degree of preserving the original structure.
It will be suspected that the tissue oxygen demand is increased in the papillary tumors but being unstable or decreased in the invasive carcinomas. A further attempt of the organ culture on tumor of the urinary bladder using this culture system is now on schedule.
