STUDIES ON FREE RADICALS IN TUMOR TISSUE OF THE URINARY BLADDER

(2) EFFECTS OF SPONTANEOUS OXYDIZATION ON ESR SPECTRA

Abstract

The clear differences of ESR spectra between normal mucosa and transitional cell carcinoma of human urinary bladder was described in the previous report of this series. In the present study the results were reconfirmed with the increased number of cases (11/13) and materials (23/25).
The materials were stored in a freezer at -20°C for a long period and the effects of spontaneous oxidization of ESR spectra were studied and the following results were obtained.
(1) The degree of spontaneous oxidization was classified into the following four types by the quality and quantity of Fe³⁺ ions as the indicator.
Type 1. The absorption of Fe³⁺ is not clearly identified and the g-value is approximately 2.009±0.001. Type 2. The absorption of Fe³⁺ is clear and the g-value is 2.023±0.002. Type 3. The absorption of Fe³⁺ is remarkablly enlarged and the g-value moves to 2.023±0.007. Type 4. The absorption of Fe³⁺ disappears. Meanwhile, the effects of spontaneous oxidization on absorptions other than Fe³⁺ which is mainly hfs due to ¹⁴N; were analyzed.
Type 1. As mentioned in the previous report, the main difference of ESR spectra between normal mucosa and transitional cell carcinoma is that the absorption of hfs due to ¹⁴N; appears in the latter but not in the former. Type 2. Absorptions of ¹⁴N;-hfs are recognized in both normal mucosa and transitional cell carcinoma. Also the absorptions are three to five times larger than that of Type 1. Type 3. The absorptions increase in both normal mucosa and transitional cell carcinoma or they disappear in some materials. Type 4. The ¹⁴N;-hfs and all other absorptions disappear.
Absorptions without hfs are recognized as followings.
Type 1. The absorptions are extremely small in fresh materials while they increase thereafter by combination with oxygen. Type 2, 3 and 4. The absorption disappears with the increase of Fe³⁺. This was seen in all materials examined but one exceptions
(2) Transitional cell carcinomas and two of normal mucosa reached Type 2 within 8 to 15 days of storage, however, four materials from normal mucosa remained in Type 1 even after 30 days of storage Four transitional cell carcinomas stored for 3 months reached to Type 3 and 4 on each two materials respectively.
(3) A clear absorption was recognized at g=4.5±0.3 in some materials with ¹⁴N;-hfs. The absorptions of heme-Fe or non-heme-Fe in lower magnetic field which were obtained by Spin Hamiltonian are 600 and 4.3 respectively. Therefore it will be concluded that the absorption of 4.5±0.3 is unable to be heme-Fe.