A STUDY ON THE INTRARENAL DISTRIBUTION OF THE ORGANIC SUBSTANCES IN URINARY CALCULI USING IMMUNOFLUORESCEINCES METHOD

Abstract

The genesis of urinary calculi is poorly understood. It has been previously reported that the compoments of the organic substances in urinary calculus are chiefly mucoprotein and mucopolysaccharide.
Actually, the patients of urinary calculi excrete mucoprotein more significantly than normal persons.
Therefore it is obvious that the organic substances, especially mucoprotein, play an important role in the genesis of urinary calculi.
The organic substances were extracted from oxalate calculi and phosphate calculi.
These organic substances were injected intramusclarily in rabbits for the formation of antiorganic substances serums.
These anti-serums were labelled with F. I. T. C. using Kawamura's technique.
The kidneys of stone formers as well as of non-stone formers were stained with this fluorescent antibody solution.
In addition, the urinary mucoprotein was extracted from the patients of urinary calculi and from normal persons and anti-urinary mucoprotein serums were obtained with these urinary mucoproteins.
Fluorescent antibody solution of urinary mucoprotein was also obtained using Kawamura's technique.
The kidneys of stone formers and of non-stone formers were stained with this fluorescent antibody solution.
Following results were obtained;
1) In the kidneys of stone formers, mucoprotein was contained more abundantly in renal medulla than in renal cortices.
2) In the kidneys of stone formers, mucoprotein was especially increased in the epithelial cells of uriniferous tubules, in intraranal spaces of uriniferous tubules and parietal layers of Bowman's capsules. These facts could be not observed in the kidneys of non-stone formers.
3) In the epithelial cells of uriniferous tubules, mucoprotein was localized only in cystoplasms and not in nucleuses. Mucoprotein was also scattered in glomeruli.
4) Regarding the intrarenal distribution of mucoprotein, there were no differences between the kidneys of oxalate stone formers and those of phosphate stone formers.
5) Immunofluorescently, urinary mucoprotein of stone formers were identical with that of normal persons.
6) It seemed that mucoprotein was contained in the kidneys of stone formers more abundantly than in those of non-stone formers.
7) In the urinary calculi, mucoprotein was contained laminately among the inorganic substances.