A STUDY ON ALDOLASE ACTIVITY AND ISOZYME PATTERN OF THE BLADDER TUMOR

Abstract

Aldolase activities and isozyme patterns of mucous membrane and tumor tissue of the bladder were studied in order to investigate their enzymochemical characteristics and forecast recurrence of the tumors.
Results were as follows:
1) The average fructose-1, 6-diphosphate (FDP) activity of normal mucous membrane of the bladder was 7.6±1.3U/g protein and the value of the tumor tissues was 43.7±5.8U/g protein.
Then FDP activity of the latter is 5.8 folds higher than the former.
2) The average fructose-1-phosphate (FlP) activity of bladder tumors was 4.2±0.8U/g protein, while no FlP activities was assayed in normal mucous membrane of the bladder.
3) The average ratio of FDP/FlP was 16.3±2.0 in bladder tumors.
4) The FDP activities were higher in large sized tumors than in medium sized tumors.
5) No relationship was found between FDP activities and number, grade or stage of bladder tumors.
6) There was an increasing tendency of aldolase C fraction in large sized tumors but not in medium sized tumors.
7) No relationship was found between aldolase isozyme patterns and number, grade or stage of bladder tumors.
8) There was an increasing tendency of aldolase C fraction in bladder tumors of high FDP activities compared with those of low FDP activities.
9) No relationship was found in bladder tumors between FlP activities or FDP/FlP activity ratios and isozyme patterns.
10) No relationship was found between recurrences of bladder tumor after the operation and the FDP activities or the aldolase isozyme patterns of the tumor.
11) The aldolase A and A₃C hybrid were found in many bladder tumors, while the aldolase A and A₃B hybrid were found in normal mucous membranes. It was suggested that a disordered pattern on gene expression occurred during carcinogenesis of the bladder.