1977 Volume 68 Issue 3 Pages 225-230
Lymphocyte mediated cytotoxicity was studied, using Takasugi & Klein microassay method. Hela cells were used as target. Our method, characterized by using as target Hela cells which are cell lines derived from histologically different tumor, provides favorable environment for tissue culture and the ease with which the phenomena can be reproduced. However, because of lack of immunological specificity to the lymphocyte to be examined, there might be nonimmunological factors to be considered to draw any definite conclusion, therefore the result could be interpreted to indicate biological function of lymphocyte in general. Based upon this understanding, the cytotoxicity, expressed by MRT was compared between a group of normal persons and a group of patients with bladder carcinoma, MRT being 49.03±5.53% and 27.67±10.6%, respectively. The difference was statistically significant (p<0.025).
Then activation of the cytotoxicity by PHA stimulated lymphocytes, namely PHA induced cytotoxicity, was studied. Enhanced cytotoxicity was noted in both healthy individual and renal tumor bearing patient, but stimulation index were 1.89 in the former and 1.43 in the latter with a statistically significant difference (p<0.05). Blastogenesis of lymphocyte was determined by means of incorporation of 3H-thymidine into DNA. In the control group, base line uptake of 4, 005±908 CPM/106 viable cells was enhanced to 11, 400±4, 969 CPM/106 viable cells by stimulation of PHA. In the group of patients with renal tumor, base line uptake of 5, 028±3, 341 CPM/106 viable cells was enhanced to 6, 555±2, 815 CPM/106 viable cells without statistically significant difference, even though the stimulation index was noted to be lower compared to the control group.
